As part of a project in conjunction with the Great Lakes Bioenergy Research Center and the Joint Genome Institute, C5-6 Technologies and Lucigen Corp. isolated, characterized, and sequenced a number of new enzyme-producing aerobic organisms from thermal features. Geobacillus species were the most common aerobic organisms isolated during the cultivation of most samples. Geobacillus species were originally classified as members of the genus Bacillus, but were reclassified as a separate genus based on 16S rRNA gene sequence analysis, lipid and fatty acid analysis, phenotypic characterization, and DNA–DNA hybridization experiments. Geobacillus species have been isolated from a number of hostile environments including high-temperature oilfields, a corroded pipeline in an extremely deep well, African and Russian hot springs, marine vents, and the Mariana Trench, yet they can also be found in garden soils and hay composts. The ability of Geobacillus species to thrive in these varied and often hostile environments suggests that these species possess enzymes suitable for applications in hostile industrial environments.
Geobacillus stearothermophilus (previously known as Bacillus stearothermophilus) is a heavily studied source of xylan-degrading enzymes. Xylan-degrading enzymes characterized from Geobacillus stearothermophilus strain T-6 include two xylanases, an alpha-glucuronidase, three xylosidases, one arabinofuranosidase, and one arabinopyranosidase.
We have isolated many novel Geobacillus species from a variety of environments including hot springs and composts. We have published a number of papers detailing the features of some of these organisms.
Recently, we compared the genomes of six of the strains we isolated and showed the range of carbohydrate-degradation enzymes these interesting organisms possess.
We have cloned key enzymes involved in carbohydrate degradation from proprietary strains of Geobacillus, produced them in E. coli, then purified them by column chromatography to give >90% purity by SDS-PAGE. Enzymes are supplied as solutions in 75 mM Tris-HCl, pH 8.0 and 25% glycerol. Follow the product links to view the data sheets for the individual enzymes. The enzyme are highly thermostable and are compatible with thermostable bacterial and fungal cellulases.
Proprietary Geobacillus enzymes
Name CAZY Family Price
α-Arabinofuranosidase 1 (Ara1) GH51 $200/2 mg
α-Galactosidase 1 (Aga1) GH36 $200/0.2 mg
β-Galactosidase 1 GH42 $200/0.2 mg
β–Glucanase 1 (Bgn1) GH10 $200/2 mg
β–Glucanase 2 GH10 $200/2 mg
β–Xylosidase 1 (Xyl1) GH52 $200/2 mg
Xylanase 1 (Xyn1) GH10 $200/2 mg
Xylanase 2 (Xyn2) GH10 $200/2 mg