To better understand the cellulolytic strategies employed by different microbes, we sequenced the genomes of two highly cellulytic bacteria, the Actinobacterium Cellulomonas fimi (ATCC 484T) and the Gammaproteobacterium Cellvibrio gilvus (ATCC 13127T). An initial analysis of these genomes using phylogenetic and whole-genome comparison revealed that Cellvibrio gilvus is not a member of the Gammaproteobacteria but belongs to the genus Cellulomonas. We proposed that Cellvibrio gilvus be reclassified as Cellulomonas gilvus comb. nov. A comparative genomics analysis between these two Cellulomonas genome sequences showed that these cellulomonads do not encode cellulosomes but instead appear to degrade cellulose by secreting multi-domain glycosyl hydrolases. Despite the minimal number of carbohydrate-active enzymes compared to other known cellulolytic organisms, these Cellulomonas species were proficient at degrading and utilizing a diverse set of carbohydrates, including crystalline cellulose. In an effort to better understand the function of these enzymes, C5-6 has cloned and expressed several of the C. fimi enzymes. At 74% G+C content for their genomes, these enzymes proved difficult to clone. Subsequently, C5-6 contracted to codon optimize and synthesize genes coding for 18 C. gilvus carbohydrases. The following cloned genes are available. Note: Cellulomonas species produce glycosylated proteins, including xylanases and cellulases. The proteins described here are produced in E. coli, and do not possess native glycosylation. If you are interested in evaluating one or more of these proteins, drop us a line.
C. gilvus
Gene Activity Structure
cgi_0019 Unc.* GH9 CBM2 CBM3
cgi_0277 Unc.* CE2 CBM2
cgi_0632 xylanase/endoglucanase GH10 CBM22 CBM2
cgi_1040 xylanase/endoglucanase GH10 CBM2
cgi_1696 endoglucanase GH5 CBM2
cgi_1697 Unc.* GH6 CBM2
cgi_1891 Unc.* CBM33 CBM2
cgi_2254 Unc.* CE3 CBM2
cgi_2269 xylanase/endoglucanase GH6
cgi_2403 xylanase GH10 CE4 CBM9 CBM22
cgi_2637 endoglucanase GH5 CBM2
cgi_2669 Unc.* GH48 CBM2
cgi_2718 xylanase/endoglucanase GH6 CBM2
cgi_2723 Unc.* GH6
cgi_2958 xylanase GH30 CBM2
cgi_2963 Unc.* CBM2
cgi_3134 endoglucanase GH74 CBM2
cgi_3149 xylanase GH10 CBM2
*Uncharacterized/ no detected activity Note: Cellulomonas species produce glycosylated proteins, including xylanases and cellulases. The proteins described here are produced in E. coli, and do not possess native glycosylation. If you are interested in evaluating one or more of these proteins, drop us a line.
C. fimi
beta-Glucosidase 7 Celf_0140 GH3
Cellobiose Phosphorylase 1 Celf_0317 GH94
Xylanase 21 Celf_0374 GH11 CBM2 CE4 CBM2
Xylanase 22 Celf_0574 CBM22 CE4 CBM22 GH10 CBM9 CBM9
Xylanase 23 Celf_1271 GH10 CBM2
alpha-Arabinofuranosidase 3 Celf_1924 GH5 CBM2
Cellulase 50 Celf_1925 GH6 CBM2
alpha-Arabinofuranosidase 4 Celf_2726 GH3
beta-Xylosidase 9 Celf_2770 GH2
alpha-Arabinofuranosidase 5 Celf_2983 GH3
Curdlanase 3 Celf_3113 GH16 CBM13
Note: Cellulomonas species produce glycosylated proteins, including xylanases and cellulases. The proteins described here are produced in E. coli, and do not possess native glycosylation. If you are interested in evaluating one or more of these proteins, drop us a line.