Enzymes from Cellulomonas species

To better understand the cellulolytic strategies employed by different microbes, we sequenced the genomes of two highly cellulytic bacteria, the Actinobacterium Cellulomonas fimi (ATCC 484T) and the Gammaproteobacterium Cellvibrio gilvus (ATCC 13127T). An initial analysis of these genomes using phylogenetic and whole-genome comparison revealed that Cellvibrio gilvus is not a member of the Gammaproteobacteria but belongs to the genus Cellulomonas. We proposed that Cellvibrio gilvus be reclassified as Cellulomonas gilvus comb. nov. A comparative genomics analysis between these two Cellulomonas genome sequences showed that these cellulomonads do not encode cellulosomes but instead appear to degrade cellulose by secreting multi-domain glycosyl hydrolases. Despite the minimal number of carbohydrate-active enzymes compared to other known cellulolytic organisms, these Cellulomonas species were proficient at degrading and utilizing a diverse set of carbohydrates, including crystalline cellulose. In an effort to better understand the function of these enzymes, C5-6 has cloned and expressed several of the C. fimi enzymes.  At 74% G+C content for their genomes, these enzymes proved difficult to clone.  Subsequently, C5-6 contracted to codon optimize and  synthesize genes coding for 18 C. gilvus carbohydrases.  The following cloned genes are available.  Note:  Cellulomonas species produce glycosylated proteins, including xylanases and cellulases.  The proteins described here are produced in E. coli, and do not possess native glycosylation.  If you are interested in evaluating one or more of these proteins,  drop us a line.

C. gilvus

Gene          Activity                                              Structure

cgi_0019         Unc.*                                                 GH9 CBM2 CBM3

cgi_0277         Unc.*                                                  CE2 CBM2

cgi_0632         xylanase/endoglucanase              GH10 CBM22 CBM2

cgi_1040          xylanase/endoglucanase              GH10 CBM2

cgi_1696           endoglucanase                                GH5 CBM2

cgi_1697           Unc.*                                                GH6 CBM2

cgi_1891           Unc.*                                                 CBM33 CBM2

cgi_2254          Unc.*                                                 CE3  CBM2

cgi_2269          xylanase/endoglucanase               GH6

cgi_2403          xylanase                                            GH10 CE4 CBM9 CBM22

cgi_2637          endoglucanase                                 GH5 CBM2

cgi_2669          Unc.*                                                 GH48 CBM2

cgi_2718           xylanase/endoglucanase               GH6  CBM2

cgi_2723           Unc.*                                                 GH6

cgi_2958          xylanase                                            GH30 CBM2

cgi_2963            Unc.*                                                CBM2

cgi_3134          endoglucanase                                  GH74 CBM2

cgi_3149          xylanase                                             GH10 CBM2

*Uncharacterized/ no detected activity Note:  Cellulomonas species produce glycosylated proteins, including xylanases and cellulases.  The proteins described here are produced in E. coli, and do not possess native glycosylation.  If you are interested in evaluating one or more of these proteins,  drop us a line.

C. fimi

beta-Glucosidase 7                                  Celf_0140                       GH3

Cellobiose Phosphorylase 1                   Celf_0317                       GH94

Xylanase 21                                               Celf_0374                       GH11 CBM2 CE4 CBM2

Xylanase 22                                               Celf_0574                      CBM22 CE4 CBM22 GH10 CBM9 CBM9

Xylanase 23                                               Celf_1271                      GH10 CBM2

alpha-Arabinofuranosidase 3                Celf_1924                     GH5 CBM2 

Cellulase 50                                                 Celf_1925                      GH6 CBM2 

alpha-Arabinofuranosidase 4                Celf_2726                      GH3

beta-Xylosidase 9                                      Celf_2770                      GH2

alpha-Arabinofuranosidase 5                Celf_2983                      GH3

Curdlanase 3                                                Celf_3113                      GH16 CBM13

Note:  Cellulomonas species produce glycosylated proteins, including xylanases and cellulases.  The proteins described here are produced in E. coli, and do not possess native glycosylation.  If you are interested in evaluating one or more of these proteins,  drop us a line.