Fibrobacter succinogenes is a well-known inhabitant of the bovine rumen, where it degrades and consumes cellulose in feed. It produces succinct acid, which is utilized by other organisms in the rumen. While the importance of this organism is well known, the method it uses to degrade cellulose and the functions of many of its enzymes are not. Our group has screened the genome for biomass-degrading enzymes using shotgun libraries, sequenced the genome and cloned numerous other enzymes not captured by shotgun cloning. These enzymes are cloned and produced in E. coli, then purified by column chromatography to give >90% purity by SDS-PAGE. Enzymes are supplied as solutions in 75 mM Tris-HCl, pH 8.0 and 25% glycerol.
beta-Mannanase 5 Fisuc_0728 GH5
beta-Mannanase 3 Fisuc_0730 CBMnc GH26
Cellulase 22 Fisuc_0786 GH5
Cellulase 28 Fisuc_0860 GH77
Cellulase 14 Fisuc_0897 GH5
Cellulase 26 Fisuc_1224 GH5
Xylanase 13 Fisuc_1426 GH45
Cellulase 23 Fisuc_1523 GH5
Cellobiohydrolase 2 Fisuc_1584 GH5
Esterase 1 Fisuc_1641 CE2
beta-Mannanase 6 Fisuc_1688 CBM35 GH26
Cellulase 29 Fisuc_1751 GH3
Xylanase 14 Fisuc_1765 GH30 CBM6 CBMnc
Xylanase 24 Fisuc_1769 GH43 CBM6 CBMnc
Esterase 2 Fisuc_1771 CE1 CBM4
alpha-Galactosidase 2 Fisuc_1773 GH27 CBM6
ChBP1 Fisuc_1789 GH95 CBM6
Xylanase 09 Fisuc_1793 GH10 CBM6 CBMnc
Xylanase 07 Fisuc_1794 GH10 CBM6 CBMnc
Cellulase 30 Fisuc_1802 GH8
Cellulase 15 Fisuc_1859 GH9
Cellulase 24 Fisuc_2033 GH9
beta-Glucanase 3 Fisuc_2065 GH3
Cellulase 16 Fisuc_2081 GH51
Xylanase 10 Fisuc_2201 GH11
Cellulase 17 Fisuc_2230 GH5
Xyloglucanase 1 Fisuc_2317 GH74
Cellulase 27 Fisuc_2362 GH9
Xylanase 08 Fisuc_2442 GH11
ChBP2 Fisuc_2485 CBM6
Esterase 3 Fisuc_2534 CE6 CBM6 CBMnc
Cellulase 18 Fisuc_2579 GH8
Cellulase 31 Fisuc_2886 GH43
beta-Mannanase 4 Fisuc_2933 GH5 CBM4
Lichenase 1 Fisuc_2961 GH16 GH16
beta-Galactosidase 3 Fisuc_3049 GH2
Cellulase 25 Fisuc_3081 GH5
Cellulase 19 Fisuc_3111 CBM30 CBM30 CBM11 GH51