Trichoderma reesei cellulase have been extensively studied and characterized, both singly and as mixtures. C5-6 is proud to offer the only complete set of purified T. reesei cellulose-degrading enzymes as well as purified T. reesei xylanases, α-glucuronidase, β-glucosidase, β-xylosidase, and α-arabinofuranosidase. All enzymes except CBHI are cloned in Pichia pastoris as secreted proteins. Proteins are purified by column chromatography to >90% purity and supplied in 50 mM Na-Acetate (pH 5.0), 20% glycerol. Because CBHI is not expressed in Pichia pastoris, CBHI is purified from T. reesei fermentation broth to >90% purity and supplied in 50 mM Na-Acetate (pH 5.0), 20% glycerol.
Name Gene CAZY Family Price
Xylanase 16 Tr_120229 GH10 enquire*
beta-Xylosidase 7 Tr_121127 GH3 $200/2 mg
Cellulase 37 (EGI) Tr_122081 CBM1 GH7 $200/2 mg
Xylanase 18 Tr_123818 GH11 enquire*
alpha-Glucuronidase 2 (Agn2) Tr_72526 GH67 $200/2 mg
Cellobiohydrolase 4 (CBHII) Tr_72567 CBM1 GH6 $200/2 mg
Cellulase 39 Tr_73643 GH61** $200/2 mg
alpha-Arabinofuranosidase 2 Tr_76210 GH51 $200/2 mg
beta-Glucosidase 4 Tr_76672 GH3 $200/2 mg
Cellobiohydrolase 5 (CBHI) Tr_123989 CBM1 GH6 $200/2 mg
*These enzymes are expressed at very low levels in Pichia, and only limited quantities are available.
**GH61 family members are copper-dependent lytic polysaccharide monooxygenases that have been reclassified into family AA9 by CAZy. The enzyme also has a measurable level of hydrolytic activity.
C5-6 is the only supplier of two additional fungal enzymes that are cloned in Pichia pastoris as secreted proteins. Proteins are purified by column chromatography to >90% purity and supplied in 50 mM Na-Acetate (pH 5.0), 20% glycerol.
The secreted A. niger alpha-xylosidase has been cloned and characterized and is reported to release xylose from isoprimeverose and xyloglucan heptasaccarides.
alpha-Xylosidase Aspindraft_43342 GH31 $200/0.2 mg
The secreted α-L-fucosidase from the plant pathogenic fungus Fusarium graminearum has been cloned and characterized and is reported to release fucose from xyloglucan and synthetic substrates. The enzyme may also have applications in removal of fucose residues from mammalian glycoproteins.
alpha-Fucosidase FG11254.1 GH29 $200/0.2 mg